FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_297_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_297_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16662306
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC887590.5326933738943458No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT408000.24486406623428955No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC184160.11052491773947735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT2196750.069.553199
TATTGGC2109700.069.5293915
TTGGCGA2033800.069.4970217
GAGCTGC3459600.069.4914510
TGGCGAA2032750.069.4829818
AAATATT2195800.069.4812812
TGTCAGT895750.069.476151
GCTGCCC3455800.069.4726612
GTAAATA2200700.069.471310
CAGCACG2198200.069.465914
TAGCAGC2415500.069.441561
GCACGTA2200100.069.434566
GTCTCGG828300.069.4330911
TGAGCTG3462600.069.4211359
ATATTGG2194950.069.4152814
ATTGGCG2048150.069.4078416
CTGTACT3453900.069.4006653
TGTACTG3454900.069.391474
CCTGTAC3459750.069.3800352
GTACTGA3460700.069.358115
Produced by FastQC (version 0.11.5)