FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_296_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_296_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29096964
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2774750.9536218280367669No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2032390.6984886808121975No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA793600.27274323190556926No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC456070.1567414387287966No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT326190.11210447935392848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC8549550.069.529610
GCTGCCC8550850.069.5149412
CCTGTAC8523750.069.4844742
TGAGCTG8547900.069.472729
TGTACTG8518950.069.446174
CTGTACT8519050.069.42843
GTACTGA8529650.069.418565
TCCTGTA8526950.069.3959961
TGCCCCG8558150.069.3615714
ACTGAGC8571450.069.356977
CTGCCCC8566400.069.300513
CTGAGCT8572050.069.295778
TGTCAGT1402900.069.28651
AGCTGCC8579300.069.2844211
GTCTCGG895650.069.2762211
GCCCCGA8564000.069.220215
GTAGGTT515650.069.211928
AGTAGGT516900.069.0851757
TAGGTTG516400.069.023299
CAACGGA2633500.069.0170061
Produced by FastQC (version 0.11.5)