FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_290_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_290_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59237720
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2502830.4225061329166619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC17984500.069.7108810
GCTGCCC17983300.069.6948912
CCTGTAC17922850.069.676062
TGAGCTG17975250.069.670679
CTGTACT17908900.069.646013
TGTACTG17913550.069.6449054
GTACTGA17935500.069.627335
TCCTGTA17911100.069.6252061
ACTGAGC18018600.069.598827
TGCCCCG18003200.069.5060114
CTGAGCT18040500.069.448958
CTGCCCC18028150.069.4401713
TATTGCA4031350.069.341491
CGTTACC4770750.069.336465
GTTACCA4769100.069.328896
GCCCCGA18040150.069.3114715
AGCTGCC18083850.069.3114411
CCGTTAC4771250.069.280844
TACCATT4762100.069.2522358
CATTACT4745700.069.25107611
Produced by FastQC (version 0.11.5)