FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_287_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_287_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18058929
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2244351.2427924158736103No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC626040.3466650763176487No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA309360.17130583989781453No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT190220.1053329353030847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC5760750.069.7356910
GCTGCCC5763750.069.7256912
CCTGTAC5744600.069.688992
TGTACTG5705950.069.68774
TGAGCTG5723650.069.687519
CTGTACT5705250.069.6861653
TCCTGTA5738450.069.672771
GTACTGA5713100.069.664815
ACTGAGC5737200.069.62487
TGCCCCG5772000.069.55247514
CTGAGCT5739050.069.532238
GTAGGTT239500.069.531138
CTGCCCC5776700.069.50819413
GCCCCGA5780050.069.40655515
AGCTGCC5791000.069.3649511
GGCAGTT338450.069.3371111
TATTGCA1105600.069.256161
GTCTCGG473050.069.22948511
CAACGGA1549550.069.187871
TGTCAGT521300.069.146251
Produced by FastQC (version 0.11.5)