FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_286_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_286_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19617793
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3484021.775949007107986No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1427290.7275487104996978No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC311610.1588404975014264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC7351700.069.59215510
GCTGCCC7350350.069.5863612
TGAGCTG7344750.069.553189
CCTGTAC7328000.069.544532
TCCTGTA7315250.069.5422741
TGTACTG7320450.069.501954
CTGTACT7320200.069.498143
GTACTGA7328100.069.489095
AGCTGCC7359900.069.4865611
ACTGAGC7367350.069.4571467
TGCCCCG7355550.069.4277314
CTGCCCC7359900.069.396213
CTGAGCT7368100.069.3717048
GCCCCGA7354100.069.3619415
CGTAAAT1089300.069.333629
TATTGCA2132700.069.290561
TTGGCGA1035300.069.24502617
GTAAATA1091950.069.2102310
TCTCGGT564050.069.1796612
TGGCGAA1034400.069.173318
Produced by FastQC (version 0.11.5)