FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_281_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_281_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22783033
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1775320.7792289990538134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT1010300.069.759988
TAGCAGC4824050.069.7522661
AGTAGGT1010900.069.742817
CGTAAAT4631250.069.740559
TATTGGC4483950.069.73440615
AAATATT4624700.069.7152112
GAGCTGC5270650.069.70933510
GTAAATA4635650.069.702910
GCTGCCC5272050.069.6929712
CAGCACG4630600.069.692764
TGGCGAA4384800.069.690918
GCAGCAC4832950.069.690323
TGAGGTA1903450.069.6889951
AGGTTGT970900.069.6888410
TGAGCTG5265300.069.674639
TTGGCGA4389300.069.6618717
ATATTGG4626400.069.6502614
CCTGTAC5257250.069.642482
GTTGTAT705750.069.6419512
GCACGTA4638500.069.63976
Produced by FastQC (version 0.11.5)