FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_280_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_280_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21677870
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC771620.3559482550638047No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT368460.1699705736772109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT541700.069.546898
CGTAAAT2676050.069.4903959
GAGCTGC4851350.069.4817310
TATTGGC2579800.069.4792415
GCTGCCC4852750.069.44942512
TTGGCGA2507500.069.4329517
TGGCGAA2504200.069.42940518
AAATATT2674950.069.4246112
CAGCACG2673850.069.4107744
TGAGCTG4851200.069.402519
GTAAATA2681300.069.4024710
CCTGTAC4839900.069.3959052
GTCTCGG766050.069.3913511
GTTGTAT419950.069.390612
TGTACTG4835200.069.383094
GTACTGA4840850.069.380915
ATTGGCG2527550.069.38051616
CTGTACT4836250.069.360943
ACTGAGC4861300.069.359847
ATATTGG2676150.069.3370814
Produced by FastQC (version 0.11.5)