FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_279_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_279_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14784085
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2306501.5601236058910646No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC532550.36021843759691585No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT408500.27631064080056356No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA337770.2284686539613375No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC315870.21365542744106245No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA273580.18505034298707024No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT250900.16970952209757995No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG202930.13726246839084055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG495700.069.4690711
TATTGGC1801150.069.2927815
TGTCAGT650400.069.289491
CGTAAAT1856850.069.269179
TGAGGTA620050.069.253921
TTGGCGA1751800.069.25033617
TGGCGAA1751300.069.2361318
CAGCACG1855700.069.230054
AAATATT1856300.069.2123912
GTAAATA1859600.069.1987210
AGTAGGT344500.069.1655357
ATTGGCG1766000.069.1590116
GTTGTAT249850.069.15813412
GCACGTA1859300.069.158126
GTAGGTT344450.069.155268
GAGCTGC2032500.069.151410
ATATTGG1856500.069.1328214
TGAGCTG2031950.069.128789
GCTGCCC2031950.069.12360412
TCTCGGT495800.069.1091612
Produced by FastQC (version 0.11.5)