FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_278_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_278_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21233373
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1565460.7372639288162083No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT532440.2507562034538743No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC320750.1510593724322556No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC239470.1127800090922907No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA219430.10334203614282102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC6670650.069.47549410
GCTGCCC6669100.069.46487412
CCTGTAC6657350.069.441182
TGAGCTG6667800.069.4243559
CTGTACT6649200.069.410263
TGTACTG6649850.069.4096154
GTACTGA6656850.069.399725
TCCTGTA6652050.069.3902051
ACTGAGC6682150.069.374797
GTCTCGG720050.069.3569911
TGCCCCG6676700.069.273114
TGTCAGT746200.069.2600861
CTGCCCC6683400.069.2204113
CTGAGCT6694650.069.1872258
GCCCCGA6681150.069.1656715
TCTCGGT720400.069.0706612
GGCAGTT450900.069.0516211
CAACGGA1940950.069.027021
CTTGTCT723650.069.007128
AGCTGCC6718150.068.9780311
Produced by FastQC (version 0.11.5)