FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_275_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_275_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28048418
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3207711.143633127543949No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1046110.37296577653684426No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC967610.34497845832160656No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA511630.18240957475747832No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC493790.17604914473251218No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA413510.14742720962016467No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT332030.11837744289178805No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG296940.10586693338640346No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG293010.10446578484390814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGT1642050.069.323331
CGTAAAT2869150.069.3022849
TATTGGC2814950.069.2603915
TGGCGAA2697500.069.2103118
CAGCACG2868300.069.20914
GTAAATA2875800.069.2066510
TTGGCGA2699550.069.20573417
GTAGGTT595500.069.19958
AAATATT2870550.069.1954412
GAGCTGC2947550.069.1796310
GCTGCCC2946050.069.17564412
GTTGTAT476950.069.1697212
ATTGGCG2762650.069.1530316
TAGCAGC3163700.069.144081
GTCTCGG826600.069.126711
ATATTGG2870750.069.11503614
GCACGTA2876550.069.103086
TGAGCTG2950850.069.072499
AGTAGGT596700.069.072077
TGAGGTA1051050.069.057881
Produced by FastQC (version 0.11.5)