FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_270_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_270_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22529593
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3186931.4145528505552676No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2366781.0505205309301415No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC669300.29707593918807146No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA566230.25132722104655864No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC462270.20518346691837708No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA437160.19403812576640866No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT352050.15626114506373906No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG292150.12967389157895573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG1022500.069.32812511
CGTAAAT2905400.069.189519
AAATATT2901550.069.1763612
TCTCGGT1021750.069.1700712
TATTGGC2823300.069.1634815
TTGGCGA2722400.069.1471217
GTAGGTT593150.069.1435858
GAGCTGC5669250.069.12916610
CAGCACG2904950.069.119524
GTAAATA2910150.069.1150610
TGTCTCG1024900.069.11455510
TGGCGAA2720850.069.1093318
GCTGCCC5667250.069.10847512
GCACGTA2910650.069.0791556
ATTGGCG2743200.069.0641216
TGAGCTG5671500.069.054229
ATATTGG2904700.069.04471614
CCTGTAC5661250.069.031962
GTTGTAT516850.069.0306712
CTGTACT5655250.069.030443
Produced by FastQC (version 0.11.5)