FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_269_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_269_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18555206
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC5089492.742890593615614No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1479700.7974581365466921No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1167980.6294621574128576No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC841900.4537271103322701No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA795550.42874759784396893No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA608270.32781635515121743No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT587760.3167628535086056No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG546970.2947798046542841No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG314720.1696127760586436No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG239720.12919285293841523No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC229730.12380891917880081No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG205880.1109553836265682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4337900.069.4897712
GAGCTGC4341300.069.4811110
CCTGTAC4332800.069.419532
TGAGCTG4340450.069.410869
TGTACTG4327900.069.4052354
CTGTACT4328150.069.384563
GTACTGA4333700.069.384235
ACTGAGC4351250.069.356827
TCCTGTA4336900.069.244121
TGCCCCG4347000.069.2177114
CTGCCCC4348850.069.20615413
GTAGGTT249200.069.198148
CTGAGCT4361850.069.115268
GGCAGTT287100.069.0967711
GTCTCGG497500.069.09135411
TGTCAGT950350.069.089471
GCCCCGA4351150.069.0833115
AGTAGGT250000.069.046717
AGCTGCC4371150.068.98440611
CCGTTAC1860000.068.93274
Produced by FastQC (version 0.11.5)