FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_264_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_264_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23521690
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC13628695.794094727037045No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2831081.2036039927403175No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1765900.7507538786541273No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1731000.7359165094004725No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1411820.600220477355156No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1200300.5102949660504836No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1091830.4641800822985083No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG762180.3240328394770954No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA754610.32081453330946885No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA453770.192915560063924No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC391860.16659517237069274No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG279820.118962540531739No Hit
CGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTA245400.10432923824776195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5649000.069.3480912
GAGCTGC5651750.069.33417510
TGAGCTG5636500.069.276499
CCTGTAC5635250.069.2727662
TCCTGTA5628950.069.2644651
TGTACTG5618550.069.249194
CTGTACT5617750.069.242753
GTACTGA5626700.069.210475
ACTGAGC5654350.069.14637
TGCCCCG5654600.069.1412414
CTGCCCC5664950.069.0569313
CTGAGCT5658850.069.021438
GCCCCGA5657400.069.00989515
GTCTCGG560550.068.9684211
CGTTACC1845150.068.960355
AGCTGCC5682800.068.95225511
GTTACCA1845150.068.930156
TGTCAGT858500.068.898441
CCGTTAC1846150.068.892664
CATTACT1838900.068.8528711
Produced by FastQC (version 0.11.5)