FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_262_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_262_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27512564
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2980351.0832687204289648No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2866041.0417204299824618No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC563220.2047137446004669No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC424280.1542131805672492No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA364800.13259396688727376No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT362100.131612597066562No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA316580.1150674288299702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC10585300.069.64439412
GAGCTGC10587000.069.6314310
CCTGTAC10546200.069.612192
TCCTGTA10535200.069.596481
CTGTACT10516000.069.5847553
TGTACTG10518100.069.583944
GTACTGA10529000.069.576735
TGAGCTG10562500.069.575119
ACTGAGC10579100.069.5496447
TGCCCCG10591100.069.5074514
AGCTGCC10612400.069.4573111
CTGCCCC10600500.069.4557213
CTGAGCT10586950.069.4329458
GCCCCGA10595950.069.4085815
GTCTCGG782200.069.2605511
CCGAGAA6228400.069.2314318
TCTCGGT780100.069.213712
CGAGAAC4738200.069.1907519
TATTGCA1925700.069.0911
CGTAAAT806100.068.9782649
Produced by FastQC (version 0.11.5)