FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_253_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_253_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29878416
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2719600.910222282198628No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC374390.1253045007472953No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA340210.11386480461347082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC8676800.069.7109610
GCTGCCC8679000.069.6973312
CCTGTAC8652150.069.665332
TGAGCTG8669800.069.657049
TGTACTG8641400.069.656624
GTACTGA8650450.069.64165
CTGTACT8643300.069.630213
ACTGAGC8686350.069.627777
TGCCCCG8693400.069.4824414
CTGAGCT8701950.069.4253168
GTAGGTT548050.069.42418
TCCTGTA8670650.069.420191
CTGCCCC8707550.069.39365413
GCCCCGA8705100.069.3464315
AGCTGCC8730800.069.2617711
GTCTCGG856350.069.2387811
GGCAGTT568350.069.2353711
GGGCTAT358950.069.22867615
CGTAAAT1039550.069.2212459
AGGTTGT531100.069.2081710
Produced by FastQC (version 0.11.5)