FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_246_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_246_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14705558
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1297280.8821698571383689No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT495750.33711743546215656No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC193840.13181410729195042No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC160580.10919680844480706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC6049200.069.7026610
GCTGCCC6043800.069.7006512
TCCTGTA6026550.069.662741
CCTGTAC6033950.069.658772
TGAGCTG6047800.069.6568769
TGTACTG6028700.069.641544
CTGTACT6028850.069.630323
GTACTGA6037550.069.617135
ACTGAGC6063700.069.593367
TGCCCCG6048500.069.54928614
CTGCCCC6050900.069.5251713
AGCTGCC6062100.069.5214311
CTGAGCT6066650.069.468368
GCCCCGA6052300.069.4657115
GTCTCGG493050.069.4168111
TCTCGGT491250.069.3719312
CCGAGAA3412350.069.26987518
TATTGCA1186250.069.2551041
CGAGAAC2599500.069.19533519
TGTCTCG494650.069.0719910
Produced by FastQC (version 0.11.5)