FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_22_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_22_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24091804
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC13650065.665852171136707No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2846191.181393473066608No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2763581.1471038034345622No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1935000.8031777113909776No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1571850.6524418013694615No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1412450.5862782214233521No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1339360.5559401031155657No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1163660.48301073676342376No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG649520.2696020605181746No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC536570.22271889643465473No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGGGTCGATGAAGAACGCAGC468950.19465125982263512No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG379860.1576718787850009No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA375840.1560032615241266No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA243370.10101775691019237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG585300.069.0300811
TGTCAGT1351500.069.026161
CAACGGA2173350.068.87621
GCTGCCC2855400.068.8711312
GAGCTGC2865350.068.7040510
TGTCTCG587750.068.694710
GTAGGTT230550.068.647718
CCTGTAC2863350.068.636672
CTGTACT2860500.068.61663
GGCAGTT338850.068.6044111
TGTACTG2860950.068.601944
TGAGCTG2867550.068.595199
GTACTGA2864400.068.5791855
TCCTGTA2862550.068.57881
CGTTACC2127300.068.511275
AGTAGGT231350.068.485967
CGTAAAT584650.068.4603049
CCGTTAC2128750.068.448164
TCTCGGT588650.068.44697612
GTTACCA2128400.068.444626
Produced by FastQC (version 0.11.5)