FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_227_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_227_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24846495
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT6493992.613644298723019No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1903940.7662811193289033No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA715620.2880164787830235No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT626520.25215629005217843No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC547400.2203127644361911No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC312080.12560322894637654No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG308710.12424690082041753No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA305020.1227617818931805No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG304460.122536397990944No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA277850.11182663792216971No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA275450.11086070691258465No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG257260.10353975480243793No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT256680.10330632147512153No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC248480.10000605719237261No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6792450.069.7504512
GAGCTGC6795800.069.7176110
TGAGCTG6795700.069.6580059
TCCTGTA6775000.069.647471
TGTACTG6782950.069.584424
CCTGTAC6791500.069.57622
CTGTACT6785400.069.554363
TGCCCCG6804100.069.55218514
CGTTACC2274700.069.504355
GTACTGA6795950.069.497665
CTGCCCC6810100.069.49618513
GTTACCA2274450.069.484296
CCGTTAC2275400.069.461434
AGCTGCC6820150.069.45792411
CATTACT2267250.069.4461711
TACCATT2273400.069.44018
GCCCCGA6814250.069.3920815
CTGAGCT6829250.069.3491068
TTACCAT2275050.069.337437
TGTCAGT939300.069.328451
Produced by FastQC (version 0.11.5)