FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_225_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_225_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26743331
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT978520.3658930893836673No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC371560.1389355723862521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC9729350.069.7328910
GCTGCCC9729450.069.7261912
CCTGTAC9697450.069.691012
TGAGCTG9725550.069.679539
TGTACTG9689950.069.671214
TCCTGTA9689350.069.669421
GTACTGA9700400.069.6638645
CTGTACT9690200.069.660513
ACTGAGC9743700.069.6485067
TGCCCCG9740000.069.55414
CTGAGCT9750350.069.519528
AGCTGCC9760200.069.4964711
CTGCCCC9748850.069.4933813
GCCCCGA9745850.069.43719515
TATTGCA2027250.069.284071
CCGAGAA5499300.069.2467318
GTCTCGG714750.069.2300711
CGTTACC1371300.069.1932455
CGAGAAC4202900.069.17873419
GTTACCA1371000.069.1726466
Produced by FastQC (version 0.11.5)