FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_221_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_221_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16461457
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1542220.936867253001967No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT957330.5815584853758693No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC427170.25949707853928117No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA327250.1987977127419523No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC224360.1362941324088141No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA213630.1297758758535165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT2543550.069.440139
GTCTCGG655200.069.4327811
AAATATT2540250.069.42583512
TATTGGC2465500.069.4224515
CAGCACG2542300.069.419424
TGGCGAA2395200.069.41785418
GTAAATA2546250.069.4132310
TTGGCGA2398350.069.4129917
GCACGTA2546450.069.3846366
TAGCAGC2789300.069.3826451
GCTGCCC2353150.069.33713512
ATATTGG2542750.069.3243414
ATTGGCG2421500.069.3157716
GAGCTGC2353850.069.3118410
TCTCGGT653450.069.30270412
TGTCAGT904450.069.2874151
GTAGGTT368000.069.256968
CACGTAA2551900.069.251327
GCAGCAC2800250.069.222223
TGAGGTA684200.069.215651
Produced by FastQC (version 0.11.5)