FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_220_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_220_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16693887
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC7498074.491506381946877No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1796831.0763400998221686No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1116640.6688915529379108No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA879160.5266358877354328No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT806440.483075032195917No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA782030.4684529133328864No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG643340.3853745984982407No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT400730.24004595215002955No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG380870.2281493818665479No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC324720.1945143153299169No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA220440.1320483360166509No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG189310.11340079155920968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGT1017550.069.420231
GTAGGTT455200.069.375968
GTCTCGG596450.069.3415411
GCTGCCC2918000.069.3402612
GAGCTGC2919750.069.3082910
CGTAAAT1223450.069.295019
TATTGGC1198300.069.27753415
CCTGTAC2913300.069.270222
GTTGTAT308500.069.2499812
CTGTACT2910050.069.246153
TGAGCTG2919400.069.2446759
GTACTGA2913050.069.2424555
TGAGGTA755550.069.240431
TGTACTG2910700.069.231024
CAGCACG1223150.069.221074
TCCTGTA2911800.069.212121
TTGGCGA1156400.069.20640617
ACTGAGC2926500.069.18917
TGTCTCG596650.069.1775210
TGGCGAA1156800.069.1401218
Produced by FastQC (version 0.11.5)