FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_215_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_215_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17484428
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1846551.0561111864797637No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC438270.25066304714114757No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT259310.14830911254288673No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC257430.14723387004710706No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA229860.1314655532339977No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT192980.11037249831678796No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA191130.10931441394594092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC3833050.069.42811610
GCTGCCC3832250.069.3923712
TGTCAGT943600.069.389281
TGAGCTG3832150.069.365889
TATTGGC1844150.069.36491415
CGTAAAT1890450.069.360079
CCTGTAC3827750.069.355082
GTCTCGG662150.069.3433711
TGTACTG3823100.069.335364
CTGTACT3823400.069.32453
GTTGTAT347750.069.3244812
ACTGAGC3841350.069.312737
GTACTGA3828000.069.31065
GTAGGTT431100.069.3087168
AAATATT1888750.069.3039112
TTGGCGA1771950.069.2887317
TCCTGTA3823750.069.288471
CAGCACG1890350.069.268654
TGAGGTA766900.069.266781
TGGCGAA1771250.069.23508518
Produced by FastQC (version 0.11.5)