FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_213_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_213_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22777004
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2673721.1738681698435842No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1859370.8163365120364382No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA656980.2884400424217338No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC604110.26522803438064113No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC382100.16775691833746No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG330490.14509809982032756No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA311580.13679586656787698No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT269160.11817181926121628No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG268700.11796986118104032No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA231470.10162442786593004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT3575850.069.5633859
TAGCAGC3936400.069.560021
TATTGGC3488850.069.54951515
TGAGGTA1803400.069.5480961
CAGCACG3571450.069.526534
AAATATT3572900.069.5249712
GTAGGTT1039900.069.5109258
AGTAGGT1039350.069.507297
GTAAATA3582000.069.4967110
TTGGCGA3357800.069.4947517
GTTGTAT662300.069.4759212
GCTGCCC3118000.069.4681512
GCACGTA3578900.069.466736
ATATTGG3574250.069.45547514
GAGCTGC3120000.069.4526310
TGGCGAA3356800.069.44752518
ATTGGCG3411750.069.4461516
GCAGCAC3944000.069.422783
CACGTAA3585700.069.3839957
GTCTCGG849350.069.36449411
Produced by FastQC (version 0.11.5)