FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_209_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_209_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17591723
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC6914553.9305700754837942No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1668440.9484233011172356No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1052180.598110827461301No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1009110.5736277225374683No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA938500.533489528001322No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT771320.43845619897493837No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG671980.38198646033705735No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA574790.3267388873733403No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG280270.15931924348740598No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC258600.1470009503901352No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG232150.13196547035216505No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG194960.11082484643488305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4269800.069.3453712
GAGCTGC4274800.069.3248410
TGTCAGT1259700.069.3064961
CCTGTAC4263100.069.285442
TCCTGTA4257900.069.263171
TGAGCTG4272900.069.255939
TGTACTG4257850.069.253664
GTACTGA4262900.069.242235
CTGTACT4258450.069.2270053
ACTGAGC4280400.069.215547
TGCCCCG4278000.069.0970914
CTGCCCC4283900.069.0174513
GTCTCGG508000.069.0134411
CTGAGCT4292600.068.9552158
GCCCCGA4283250.068.9511115
GGCAGTT311550.068.92020411
GTAGGTT212050.068.8930058
AGTAGGT212500.068.8788767
TAGGTTG211500.068.8404859
AGGCAGT312950.068.70135510
Produced by FastQC (version 0.11.5)