FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_208_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_208_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18939900
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3578681.8894925527589903No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1290150.681180998843711No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1024580.5409637854476529No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC592760.3129689174705252No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA555920.2935179172012524No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA448330.23671191505762967No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT403350.2129631096257108No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG392420.20719222382377944No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC191240.10097202202757143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT579700.069.286448
GTCTCGG739150.069.2744111
CGTAAAT2210550.069.226229
TATTGGC2151450.069.2033815
TTGGCGA2088150.069.193417
TGAGGTA983000.069.1896441
GTTGTAT415050.069.1893412
TGGCGAA2086950.069.1627418
AGTAGGT581050.069.1616067
AAATATT2210250.069.1580312
CAGCACG2212650.069.1489644
ATATTGG2210350.069.1374814
GTAAATA2215850.069.1364610
GCACGTA2214250.069.108486
GCTGCCC3696900.069.1061112
ATTGGCG2112250.069.09286516
GAGCTGC3703000.069.06599410
TGTCTCG742050.069.0083910
CCTGTAC3695300.069.0069352
TGAGCTG3704350.068.986029
Produced by FastQC (version 0.11.5)