FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_1_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_1_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12444685
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC8203216.591737757926376No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1434801.1529419989336813No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1053510.8465541715198095No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT948390.7620843757797003No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA795100.6389072925509967No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT613350.4928610085349689No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA590880.4748051075619833No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG588640.47300514235595353No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG424830.34137465110607457No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC262810.21118252490922831No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG235930.18958294243687165No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA217840.17504661628639054No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG156410.1256841776228165No Hit
CGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTA125230.10062930479959918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC3157350.068.94900512
GAGCTGC3160800.068.9180410
CCTGTAC3153150.068.914542
CTGTACT3148650.068.8963553
TGTACTG3149450.068.893584
TCCTGTA3150400.068.876911
GTACTGA3152900.068.8625955
TGAGCTG3159450.068.854439
ACTGAGC3165900.068.84467
TGCCCCG3161450.068.69684614
CTGCCCC3164250.068.6847213
CTGAGCT3169550.068.673678
GTCTCGG313650.068.6262311
GCCCCGA3160750.068.6090815
TGTCAGT345050.068.413991
AGCTGCC3186350.068.3478311
TGTCTCG315450.068.1902610
TCTCGGT314600.068.1853712
CCGAGAA1494850.068.12623618
GTAGGTT78900.068.091318
Produced by FastQC (version 0.11.5)