FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_185_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_185_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960460
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC91090.9483997251317078No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT60630.6312600212398225No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC21430.22312225391999665No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA13220.13764237969306375No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC13160.13701767902879872No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA10220.10640734647981176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTA900.070.007081
GTTTCTT150.002224819369.99979446
GGTAACC150.002224819369.99979443
GAATCGG150.002224819369.99979446
GTCTAGG150.002224819369.99979447
AGAGTAT150.002224819369.99979442
CAGGGAT2000.069.99979411
AGGTTTT150.002224819369.99979445
ATTGGAT301.0241274E-769.99979440
CATAAGT150.002224819369.99979444
CAGCTGG150.002224819369.99979447
GTTGGGT150.002224819369.99979459
GTTGTAT14800.069.763312
TAGCAGC149050.069.67831
TTGGCGA132550.069.65652517
CAGCACG139950.069.6496664
TATTGGC135950.069.63936615
GTAGATT8900.069.606538
ATTGGCG134250.069.5826616
ATATTGG140050.069.5749414
Produced by FastQC (version 0.11.5)