FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_177_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_177_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22050724
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC7504603.4033349653281224No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3226931.46341226709835No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1824110.8272336092003146No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC962350.43642557949571176No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA809990.36733034253206376No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG791000.35871838040329196No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT461750.2094035551848547No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA441240.20010227328590208No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG330760.14999960999013004No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG310280.1407119330866415No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA251890.11423207691502554No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG227900.10335261554223797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5471000.069.5284612
GAGCTGC5472550.069.52347610
CGTAAAT3318000.069.520979
TAGCAGC3429950.069.511291
CAGCACG3314900.069.502314
TATTGGC3239100.069.497315
GTAGGTT638250.069.4943168
TTGGCGA3152500.069.4917617
TGGCGAA3150750.069.4859318
AAATATT3314000.069.4748312
TGAGCTG5471100.069.472979
TGAGGTA1256600.069.4687041
GTAAATA3322900.069.4583310
ATATTGG3316650.069.436214
GCACGTA3320850.069.433646
CCTGTAC5463000.069.431192
ATTGGCG3173850.069.42995516
TGTACTG5456450.069.4203954
CTGTACT5457950.069.397913
GTACTGA5465100.069.394415
Produced by FastQC (version 0.11.5)