FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_170_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_170_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24390782
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC15389346.309490200027207No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC3442341.4113282632758557No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC2015020.8261399736999002No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1985000.8138320452374179No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1376770.5644632468118489No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1227610.5033089959969304No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT960130.3936446154124948No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA772010.31651711699936474No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG711470.2916962645970105No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA538970.22097282489753708No Hit
CGACTCTTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGATCATC411860.1688588746355078No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG407630.16712461289679026No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA347440.1424472573286088No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC334020.1369451787154672No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG303660.12449785332835989No Hit
TCGTACGACTCTTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGA260030.10660994797132785No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG248940.10206314828282259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4550800.069.1967912
CGTAAAT2319500.069.188669
GAGCTGC4555050.069.1637310
TGGCGAA2205300.069.1471218
CAGCACG2320300.069.133974
TTGGCGA2208500.069.1103117
TATTGGC2268200.069.1055215
GTAAATA2324700.069.0820610
AAATATT2319100.069.07532512
TGAGCTG4555450.069.0693059
GCACGTA2324350.069.069226
ATATTGG2318500.069.0252714
CCTGTAC4547950.069.0076142
ATTGGCG2228850.068.9990916
TGTACTG4543550.068.9899144
GTAGGTT418050.068.9859168
TCCTGTA4542100.068.976261
CTGTACT4543800.068.971013
TGCCCCG4558400.068.936314
GTACTGA4552650.068.92125
Produced by FastQC (version 0.11.5)