FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_169_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_169_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10486446
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC4823104.5993656954892055No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3217063.067826792795195No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1083391.0331336279231305No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC645650.6156995420564794No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG468050.44633806343922433No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA434310.4141631969496624No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA331160.31579812645771504No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT282360.26926186431513593No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG227320.21677506373465327No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA163800.15620163399496836No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG126510.1206414451569197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG293300.069.2589811
TGAGGTA705700.069.221471
TCTCGGT292050.069.2078812
GTTGTAT259000.069.1880312
GTAGGTT402400.069.129398
TATTGGC1463800.069.0902515
CGTAAAT1501400.069.090029
TTGGCGA1422150.069.0867717
CAGCACG1502150.069.06554
AAATATT1500250.069.04712
GTAAATA1503050.069.0464410
AGTAGGT402900.069.0435947
TGTCTCG294200.069.0352110
TGGCGAA1422500.069.02302618
ATTGGCG1432550.069.0075616
GCTGCCC2494200.068.97867612
ATATTGG1501550.068.9755614
TAGGTTG403200.068.974869
GCACGTA1506050.068.9679956
GAGCTGC2495800.068.963910
Produced by FastQC (version 0.11.5)