FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_162_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_162_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21261940
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC11386895.355527294310868No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC3120191.4675001434488104No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3007241.414377051200408No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1429750.6724456940429706No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1384450.651140018267383No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1270160.5973866919011153No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1236630.581616729235432No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT796430.3745801182770716No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG539880.25391850414402445No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT461640.21712035684420145No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA403830.18993092822197785No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA364130.17125906667030386No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA243680.11460854465773114No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG241080.11338570233948549No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5912700.069.65430512
GAGCTGC5916300.069.6415110
TGAGCTG5913250.069.595749
CCTGTAC5905650.069.5271152
TGTACTG5901800.069.499614
CTGTACT5900350.069.494923
GTACTGA5909150.069.489565
TGCCCCG5921250.069.4786614
AGCTGCC5930800.069.4511711
ACTGAGC5936550.069.422237
CTGCCCC5927400.069.404813
GCCCCGA5928200.069.3434815
GTTACCA1314750.069.238776
CTGAGCT5947050.069.2290348
CGTTACC1315800.069.220765
CCGTTAC1316650.069.144174
CATTACT1303550.069.0886211
TACCATT1309500.069.071378
TCCTGTA5937150.069.039121
CCGAGAA3208050.069.0185118
Produced by FastQC (version 0.11.5)