FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_155_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_155_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24671469
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT2815331.141127834747092No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC782630.3172206729968126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT435900.069.669438
GAGCTGC6234200.069.6596810
GCTGCCC6230450.069.65330512
TGAGCTG6235650.069.615289
CCTGTAC6221750.069.595782
TCCTGTA6217250.069.594911
TGTACTG6219300.069.5816354
CTGTACT6218450.069.572863
CGTAAAT2487200.069.5553749
GTACTGA6229300.069.5445255
ACTGAGC6254750.069.5034267
AAATATT2488000.069.4937612
GTAAATA2493300.069.480810
TGGCGAA2342200.069.4805418
TATTGGC2407550.069.4710815
TAGCAGC2695150.069.470931
GCACGTA2490650.069.4305046
AGTAGGT437350.069.430457
CTGAGCT6257950.069.422028
TTGGCGA2345700.069.42013517
Produced by FastQC (version 0.11.5)