FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_14_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_14_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23731331
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2366430.9971754218084102No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC455580.19197406163185707No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC315250.13284126372852834No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA303050.12770038056441083No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT279210.1176545892010861No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA247160.10414923629862985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT3410300.069.581959
TATTGGC3306950.069.5467115
TTGGCGA3197400.069.5300517
GAGCTGC4226250.069.50587510
TAGCAGC3757700.069.4993061
GTAAATA3416500.069.4976710
CAGCACG3409250.069.4886864
TGGCGAA3196550.069.4839418
AAATATT3410600.069.48244512
GCTGCCC4225950.069.4685712
GCACGTA3413050.069.462596
GCAGCAC3763600.069.455723
TGAGCTG4227700.069.438169
CCTGTAC4220350.069.431912
ATTGGCG3228400.069.43127416
ATATTGG3411750.069.4138914
CTGTACT4217650.069.4123153
TGTACTG4219100.069.403494
GTACTGA4223400.069.3966455
AGCACGT3413500.069.39095
Produced by FastQC (version 0.11.5)