FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_149_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_149_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20428941
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1008930.493872883572379No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT555690.27201116298686256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG774450.069.4021611
TATTGGC3237350.069.3565215
CGTAAAT3334750.069.350099
TTGGCGA3150900.069.3149817
CAGCACG3333150.069.3091354
AAATATT3331650.069.29275512
TGGCGAA3148000.069.287518
GTAAATA3341150.069.2642210
ATTGGCG3178750.069.2589716
TAGCAGC3585400.069.254661
GTTGTAT488050.069.25288412
ATATTGG3333100.069.2426714
GCACGTA3338450.069.242086
GAGCTGC2502850.069.21683510
TGAGGTA1141200.069.2144851
TGTCAGT719050.069.195411
GTAGGTT599950.069.187818
GCTGCCC2502100.069.1692212
GCAGCAC3593050.069.1650543
CACGTAA3343700.069.1508567
Produced by FastQC (version 0.11.5)