FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_148_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_148_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18986542
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC7400803.897918852205947No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1547470.8150351970358793No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1144210.602642650778641No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA925400.4873978631811943No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT853160.4493498605485928No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG650780.34275857078134603No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT574360.3025090087494606No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA520680.27423635120076106No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC347300.1829190381271113No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG332240.17498710402347095No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA269390.14188470970648578No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA190130.10013935133633076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG762000.069.1766611
GTAGGTT288450.069.149478
GCTGCCC3424050.069.1013612
GAGCTGC3430550.069.0255210
TCCTGTA3425300.068.958611
TGAGCTG3431550.068.937079
CCTGTAC3431650.068.928952
CGTAAAT1425150.068.918259
CAGCACG1423550.068.915124
CTGTACT3428350.068.901623
TCTCGGT760800.068.8993412
TGTACTG3429550.068.8767854
TAGGTTG289000.068.8604359
GTTGTAT209050.068.8603512
TTGGCGA1342250.068.8548417
GTACTGA3434600.068.851945
TATTGGC1382050.068.84656515
AAATATT1424800.068.83446512
TGTCTCG764650.068.8316410
GCACGTA1428000.068.79356
Produced by FastQC (version 0.11.5)