FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_146_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_146_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20664101
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC5872082.841681813305113No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1695270.8203937834024331No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1504840.7282387944193652No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA970940.46986800925914946No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC904720.4378220954301375No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA720300.3485755320301619No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT604370.29247340593234616No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG545470.26396986735595224No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG352850.17075506938337168No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG281650.13629917894807037No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG261760.12667379045427624No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC223700.10825537486484411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT546000.069.42858
TATTGGC2053200.069.4192615
CGTAAAT2114000.069.393059
CAGCACG2111650.069.381614
GTTGTAT394150.069.3774212
GCTGCCC3643750.069.3592812
TGAGGTA976000.069.352031
TTGGCGA1991950.069.3509817
AGTAGGT547100.069.346477
GAGCTGC3645750.069.3308310
TGGCGAA1989650.069.3256118
AAATATT2112700.069.31978612
GTCTCGG564650.069.3171711
GTAAATA2118350.069.2885510
GCACGTA2118000.069.279356
TGAGCTG3647100.069.259119
ATTGGCG2016450.069.2524916
ATATTGG2114550.069.2291714
TGTACTG3637500.069.2175754
CCTGTAC3642100.069.2132
Produced by FastQC (version 0.11.5)