FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_139_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_139_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283946
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC10450.36802772358124436No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT5450.19193790368591213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGC207.9116435E-570.011981
GCTGGTC353.6943675E-970.011982
TGAGGGG252.8326012E-670.011981
GAGGGGC252.8326012E-670.011982
TCAGTGC150.00222166670.011981
TATTTGC207.9185505E-569.999659
TGATAAT207.9185505E-569.9996521
GTTTAGT207.9185505E-569.9996520
TAGGTTG1950.069.999659
TAGGTTA207.9185505E-569.9996517
GTAGGTT1950.069.999658
GAGAGCG252.835568E-669.9996510
GTAATTC207.9185505E-569.999656
GTGTGGT455.456968E-1269.9996515
AGTGCTG900.069.999654
CTGTTCG900.069.999658
CCCTTGT353.6998244E-969.999659
AGGGGCA252.835568E-669.999653
GCTGTTC900.069.999657
AGTAGGT1950.069.999657
Produced by FastQC (version 0.11.5)