FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_135_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_135_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19542375
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2888761.4782031354940226No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1702610.871240061660878No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC654930.33513326809049565No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA598800.30641106825552167No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA448730.2296189690352375No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC446680.2285699665470548No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG307690.15744759784826562No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT282920.14477257753983333No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG219750.11244794964788056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT1081000.069.4817
TGAGGTA1739550.069.479551
CGTAAAT2770000.069.4681859
GTTGTAT856700.069.4634912
GTAGGTT1081250.069.455238
TATTGGC2702000.069.4259715
TTGGCGA2603950.069.3953617
CAGCACG2769400.069.3808444
GAGCTGC3783550.069.35947410
GCTGCCC3782550.069.35931412
GTAAATA2776850.069.3558710
AGGTTGT1022100.069.3549210
AAATATT2773850.069.3274212
TGGCGAA2603500.069.3240118
ATTGGCG2635200.069.3224716
TAGCAGC3181700.069.316911
TGAGCTG3782100.069.290019
GCACGTA2775650.069.2889256
ATATTGG2773300.069.2879914
CCTGTAC3775200.069.273512
Produced by FastQC (version 0.11.5)