FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_126_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_126_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19351406
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC4431292.289905963421986No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1116650.5770381749005732No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT812090.4196542618143612No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA671510.34700837758248676No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC666030.3441765420042347No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT478020.24702081078759858No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG422980.21857843300895036No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA313830.16217426268664922No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC222360.11490637941243133No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG197020.10181172365460163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4962200.068.96545412
GAGCTGC4967850.068.946910
TGTCAGT797500.068.911151
CCTGTAC4955850.068.9019552
GTCTCGG598500.068.89934511
TGAGCTG4965100.068.8976759
TGTACTG4950150.068.871384
CTGTACT4950900.068.865383
TCCTGTA4953300.068.857561
GTACTGA4956950.068.8517465
ACTGAGC4977400.068.827977
TGCCCCG4964650.068.7798514
CATTGCA601150.068.724531
CTGCCCC4977450.068.6423513
GCCCCGA4969900.068.6064815
CTGAGCT4993250.068.549918
TGTCTCG601500.068.5033410
TCTCGGT599700.068.4521612
CAACGGA1823650.068.435551
AGCTGCC5018700.068.2350711
Produced by FastQC (version 0.11.5)