FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_125_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_125_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18788067
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC11313796.021795642947196No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2826831.5045879919419065No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1837690.978115524071742No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1772820.9435882893115082No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1339350.7128726973349627No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1098750.584812689884489No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1065280.5669981909261873No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA842280.44830583156851633No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC517460.27541949898305135No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG480530.25576340557014193No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG313650.16694106956293056No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA301120.16027194282413407No Hit
CGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTA215440.1146685286996262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGT1267750.069.376331
GCTGCCC3049700.069.2676912
GAGCTGC3051600.069.21538510
GTAGGTT277550.069.179088
TGAGCTG3047900.069.138669
CCTGTAC3043250.069.135362
CTGTACT3035900.069.126533
TCCTGTA3039800.069.119391
GTACTGA3040450.069.109475
TGTACTG3037500.069.100544
GTTGTAT158250.069.0919612
ACTGAGC3054550.069.083247
AGTAGGT278200.069.055197
GTCTCGG428800.069.0192811
CGTAAAT836950.069.011849
TATTGGC818550.068.9982115
TGCCCCG3055450.068.9895814
TTGGCGA798200.068.9871417
TGGCGAA798300.068.93465418
GTAAATA838800.068.91804510
Produced by FastQC (version 0.11.5)