FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_124_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_124_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14370800
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC9596686.677902413226821No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2170881.5106187546970244No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1505301.0474712611684807No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1225730.852930943301695No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA920720.640688061903304No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG861810.599695215297687No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT604400.4205750549725833No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG459530.31976647089932364No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA411240.2861636095415704No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA334160.2325270687783561No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC247040.17190413894842319No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG162150.1128329668494447No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA143740.10002226737551145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC3754950.069.150112
GTCTCGG284000.069.1365711
GAGCTGC3758450.069.09688610
TGAGCTG3749000.069.04919
CCTGTAC3747950.069.0159152
TGCCCCG3756350.069.008814
TGTACTG3735900.068.998954
CTGTACT3735850.068.9971163
TCCTGTA3742850.068.99211
GTACTGA3741500.068.9702765
CTGCCCC3761650.068.93947613
GTAGGTT209600.068.9140858
CGTAAAT890750.068.856089
ACTGAGC3763250.068.8536767
GCCCCGA3761500.068.8473215
AGCTGCC3772200.068.8292511
CAGCACG890500.068.82924
CTGAGCT3761300.068.827038
GTAAATA892100.068.7947910
TGGCGAA853650.068.78564518
Produced by FastQC (version 0.11.5)