FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_11_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_11_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13461995
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC4724033.509160417902399No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC895300.6650574450517921No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT814480.605021766833222No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC666660.4952163479484281No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA643310.47787122191027404No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT495210.36785781007941243No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA493700.3667361338345468No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG450720.33480921661313945No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC158620.11782800394740899No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG147190.10933743475614126No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG144130.1070643689884003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT376600.069.2736368
GTCTCGG414250.069.27193511
GGGCTAT225250.069.2371715
TGAGGTA713850.069.200561
GTTGTAT291150.069.1931212
AGTAGGT377500.069.164117
TATTGGC1906750.069.1266515
CGTAAAT1968100.069.1040349
AAATATT1965900.069.0798912
CAGCACG1965650.069.071374
TTGGCGA1839900.069.0710117
GTACAGG270900.069.0683110
GTAAATA1970650.069.0465810
TGGCGAA1838950.069.0343618
TGTCAGT614850.069.028831
GCACGTA1968450.069.017586
TCTCGGT413750.069.0003612
ATATTGG1966400.068.99824514
ATTGGCG1861600.068.99132516
CAGCATT270450.068.926473
Produced by FastQC (version 0.11.5)