FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_118_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_118_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13925526
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC6773384.864002982723957No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1596461.1464270721263958No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1218070.8747030453284135No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC806870.579417969561796No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA694880.49899730897059114No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA537100.3856945870482738No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG533910.38340382977274967No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT450690.3236430710050019No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG363890.2613114937274183No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA256620.18428029217711417No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG209180.1502133563931445No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG160050.11493282192715736No Hit
CGACTCTTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGATCATC154170.11071036024061139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGT459150.069.040781
GTCTCGG339150.068.9978611
GTTGTAT247650.068.91067512
TATTGGC2263950.068.79458615
TGAGGTA606900.068.7880551
TGGCGAA2190900.068.78393618
GGGCTAT157400.068.775915
TTGGCGA2192750.068.7753817
CAGCACG2346150.068.758454
GTAGGTT314250.068.751488
CGTAAAT2345550.068.749949
ATTGGCG2206250.068.7321116
AAATATT2343800.068.70122512
GTAAATA2348050.068.6976110
GCACGTA2349350.068.6826866
GTATAGT374900.068.6732215
TCTCGGT339300.068.6475912
ATATTGG2345450.068.6409514
GGCTATG145600.068.6294516
TGTCTCG340650.068.6221210
Produced by FastQC (version 0.11.5)