FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_108_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_108_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21337393
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC15439347.235813672270084No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT3330831.561029503463708No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC3167331.4844034601602922No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1978660.9273204088240771No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1276510.5982502173531696No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1273420.5968020554338573No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1245740.5838295240660375No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG786090.3684095803081473No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA565470.26501363123414373No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA528630.24774816679807135No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG465450.21813817648669637No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG349400.1637500888698071No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC266860.12506682517400322No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG259000.12138315116565553No Hit
TGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC243710.11421732729954405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG500350.068.92161611
GTTGTAT468550.068.8186312
GTAGGTT543550.068.801188
TCTCGGT499200.068.7017912
AGTAGGT544650.068.68157
TGAGGTA921700.068.665521
GCAATGG204600.068.665365
TTGGCGA2393050.068.63785617
TGGCGAA2390800.068.6263318
GGTTGTA469600.068.5976711
GTATAGT610000.068.5873915
CGTAAAT2554850.068.5768669
GTAAATA2556350.068.5708510
CAGCACG2554550.068.569284
TATTGGC2478650.068.5670415
TAGGTTG545000.068.566769
ATTGGCG2409750.068.56402616
TGTCTCG502300.068.55649610
AAATATT2549300.068.551812
ATATTGG2551350.068.5310114
Produced by FastQC (version 0.11.5)