FastQCFastQC Report
Fri 13 Nov 2020
HWCTGDRXX_n01_C1_100_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCTGDRXX_n01_C1_100_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17778010
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC186688510.501090954499407No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC3882262.1837427248606565No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC2263831.2733877413726284No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT1611040.9061981627864986No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1595790.8976201498367928No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1489490.8378271808824497No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1262320.7100457250277168No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1102850.6203450217431534No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG916460.5155020162549127No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA668120.3758125909480307No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG589020.3313194221400483No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC506250.28476190529761203No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG439830.24740114332256535No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAA418480.23539192519297716No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT336470.18926190276639512No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG291690.16407348179014414No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA277900.15631670811300027No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA258850.14560122308402348No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA227130.1277589561486353No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC225560.12687584268430493No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG221340.1245021236910093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG361300.069.1270511
GCTGCCC3271600.069.0531912
GAGCTGC3276400.068.9637710
TGAGCTG3271550.068.931219
GTTACCA923200.068.8960656
CGTTACC923800.068.8892065
TGCCCCG3275800.068.8684914
TCCTGTA3266700.068.849761
CCGTTAC924350.068.798994
TCTCGGT361500.068.79835512
CATTACT921300.068.76803611
CCTGTAC3276000.068.766512
TGTACTG3269600.068.754344
CTGTACT3269200.068.74963
CTGCCCC3284200.068.7125913
AGCTGCC3288950.068.6889111
GTACTGA3275500.068.684995
TACCATT924600.068.6777958
GCCCCGA3280900.068.660115
TTACCAT925600.068.626287
Produced by FastQC (version 0.11.5)