FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380973424
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG8025870.21066745065136092No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA6193780.16257774453054763No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATCACTA4073680.10692819350044742No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT3896280.10227170071579587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG409000.057.8649843
GTATGCC420600.055.9611845
CTCGTAT361950.055.8911642
TATGCCG427200.054.41658846
TCAGATT1277950.052.159012
CAGATTG1333650.050.10923
AGATTGA1353600.049.6318554
TTGAACG1354850.049.130357
ATGCCGT472800.048.3466847
GCCGTCT418700.047.59681349
TGCCGTC482450.045.03641548
CACGTCT623150.044.34324614
ACGTCTG633000.043.63089415
TAATCTC483850.043.21312738
CTGAACT654150.042.27875519
GATTGAA1604700.041.664835
GAACTCC660100.041.5106321
ACACGTC674700.041.12181513
CTTGTCT577250.040.923291
CGTATGC577450.040.74252744
Produced by FastQC (version 0.11.2)