FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26610553
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTG351190.13197395785048133No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC315900.11871230184506124No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT308850.1160629769700765No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACC293970.11047121042542783No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG285480.10728074685257387No Hit
AGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGC269750.10136955815987739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27650.044.3034146
TCGTATG30800.039.6587443
GTATGCC32450.037.9657745
CTCGTAT29350.036.84805742
CACGTCT38300.036.462314
ACACGTC40400.035.1737513
ACGTCTG40300.034.56590715
TATCTCG75300.032.212335
ATGCCGT38750.031.34166147
CGTATGC39500.031.27820644
GGATATC80500.031.0446642
CTTGTCT83400.029.4200881
ATATCTC89650.027.8370934
GATATCT91450.027.3657263
TGTTTAC82200.027.25165
GCCGTCT41950.027.11532649
AGTCACC62000.025.121628
TGCCGTC47600.024.63213548
CGGGACT61850.023.9382651
TCTCGGC103350.023.7400477
Produced by FastQC (version 0.11.2)