FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17017959
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG204590.1202200569410233No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA181710.10677543646685245No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTG179600.10553556980599141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAT31050.054.22006229
ACATTCC31650.052.97024532
CTCGTAT17200.052.90708544
TCGTATG16100.052.8261945
GTATGCC17450.052.75082447
TATGCCG17800.050.73044248
CACATTC34100.049.9857331
CACGTCT34500.049.60879514
ACGTCTG35550.048.6356815
ACACGTC36150.047.24753613
CTGAACT37300.046.63493319
GAACTCC37600.044.95940421
AGTCACA40800.042.89328
TCAGATT38550.042.673072
GCCGTCT16400.040.7620451
GAGCACA42450.040.7304739
CAGATTG41800.039.1877173
ATGCCGT21400.038.92508749
AGATTGA43950.037.5893484
TTGAACG43950.036.7121437
Produced by FastQC (version 0.11.2)