FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10048029
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAT31700.059.4007329
CACGTCT32500.058.90807314
ACACGTC33600.057.3976413
ACGTCTG33500.057.35829515
CTGAACT35100.054.64341719
ACATGAG34700.054.4661332
CACATGA35100.054.2442931
GAACTCC35750.053.747821
TGAGCAT35950.049.6518835
GAGCACA39100.049.59269
AGTCACA40700.047.89933828
GAGCATC33500.043.04441536
TCTAGTT26850.040.6720737
GTACTCT27250.040.3319443
TAGTTGT29250.037.3346949
TCTAATG30250.035.52036320
TACCTCT30300.035.46227616
CAGTCAC64000.034.50786627
TCTGAAC56500.034.38075618
AACTCCA58550.034.0135522
Produced by FastQC (version 0.11.2)