FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9309875
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT648790.696883685334121TruSeq Adapter, Index 25 (97% over 44bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA234340.25171122061252166No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT108540.11658588326910942No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG94760.10178439560144471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG75200.066.55475645
CTCGTAT71850.065.8583544
TATGCCG74750.065.6447648
GTATGCC75350.065.5865447
TGATATA79250.064.4342736
ATGCCGT76350.064.2232649
GCCGTCT74400.063.97778751
TGCCGTC76450.063.49831450
GATATAT80400.063.46911237
TATATCT75800.062.98049539
ACTGATA81750.062.89328434
CACGTCT82850.062.8605714
ACGTCTG83100.062.62867415
CGTATGC80600.061.9654946
GAACTCC83600.061.75237321
CTGAACT84500.061.67420619
ACACGTC85000.061.5604613
ATATATC79350.060.6039238
GCTTGAA75000.059.4523661
AGTCACA87500.059.44125728
Produced by FastQC (version 0.11.2)